Propofol Ameliorates Microglia Activation by Targeting MicroRNA-221/222-IRF2 Axis

Figure 5

Propofol suppresses BV2 activation via targeting miR-221/222-IRF2 axis. (a) IRF2 protein expression in LPS-primed BV2 microglia upon treatment with propofol and/or miR-221/222 mimics was analyzed by Western blotting; β-actin was loaded as a control. (b) The effects of propofol and/or miR-221/222 mimics on the mRNA expression of inflammatory genes (Il1b, IL6, Tnf, Ptgs2, and Nos2) in LPS-primed BV2 cells were determined by real-time qPCR analysis (). (c) The effects of propofol and/or miR-221/222 mimics on the level of inflammatory cytokines (IL-1β, IL-6, and TNF-α) in conditioned medium from LPS-primed BV2 cells were analyzed by ELISA (). (d) IRF2 protein expression in LPS-primed BV2 microglia upon treatment with propofol and/or siRNAs against IRF2 was analyzed by Western blotting; β-actin was loaded as a control. (e) The effects of propofol and/or IRF2 siRNAs on the mRNA expression of inflammatory genes (Il1b, IL6, Tnf, Ptgs2, and Nos2) in LPS-primed BV2 cells were determined by real-time qPCR analysis (). (f) The effects of propofol and/or IRF2 siRNAs on the level of inflammatory cytokines (IL-1β, IL-6, and TNF-α) in conditioned medium from LPS-primed BV2 cells were analyzed by ELISA (). and .

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