Propofol Ameliorates Microglia Activation by Targeting MicroRNA-221/222-IRF2 Axis

Figure 6

Propofol-miR-221/222-IRF2 axis is involved in primary microglia activation. (a) miR-221 and miR-222 expression in primary microglia upon treatment with LPS and/or propofol were analyzed by real-time qPCR (). (b) IRF2 protein expression in primary microglia upon treatment with LPS and/or propofol was analyzed by Western blotting; β-actin was loaded as a control. (c) IRF2 protein expression in LPS-primed primary microglia upon treatment with propofol, miR-221/222 mimics, and/or IRF2 siRNAs was analyzed by Western blotting; β-actin was loaded as a control. (d) The effects of propofol, miR-221/222 mimics, and/or IRF2 siRNAs on the mRNA expression of inflammatory genes (Il1b, IL6, Tnf, Ptgs2, and Nos2) in LPS-primed primary microglia were determined by real-time qPCR analysis (). (e) The effects of propofol, miR-221/222 mimics, and/or IRF2 siRNAs on the level of inflammatory cytokines (IL-1β, IL-6, and TNF-α) in conditioned medium from LPS-primed primary microglia were analyzed by ELISA (). and .

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