Research Article

Duck Interleukin-22: Identification and Expression Analysis in Riemerella anatipestifer Infection

Figure 3

mRNA expression profiles of duck IL-22 in splenic lymphocytes and macrophages. Lymphocytes and macrophages were collected and isolated from 2-week-old healthy ducks and treated with heat-inactivated R. anatipestifer serotype 7 for the indicated times. RNA was isolated and samples were applied to qRT-PCR analysis. The transcript expression levels of IL-22 (a) and IL-17A (b) were normalized to expression levels of β-actin used as a reference gene and presented relative to the expression levels of untreated/cultured lymphocytes and macrophages (NC). Results are presented as the values from 3 independent experiments performed in triplicate. and . RA: R. anatipestifer-stimulated lymphocytes.
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