Duck Interleukin-22: Identification and Expression Analysis in Riemerella anatipestifer Infection

Figure 5

IL-22 is produced primarily by CD4⁺ cells during R. anatipestifer infection, but not IL-17. The spleens were collected from 2-week-old healthy ducks. Spleen cells were isolated, cultured, and stimulated with heat-killed R. anatipestifer serotype 7. After 24 h, cells were collected, labelled, and separated following incubation in MACS buffer. Total RNA was obtained, and qRT-PCR analysis was performed. Gene expression levels of IL-22 (a) and IL-17A (b) transcripts were normalized with β-actin used as a reference gene and calibrated with the expression levels of untreated cultured spleen cells (NC). These results represented one representative of 2 independent experiments. Results are presented as the values performed in triplicate. . RA: splenic lymphocytes stimulated with heat-inactivated R. anatipestifer.

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