Duck Interleukin-22: Identification and Expression Analysis in Riemerella anatipestifer Infection

Table 1

Primers used for cloning and expression analysis.

Target genes	Purpose	Orientation and sequence (5-3)	References
DuIL-22	3 RACE	(For) TACCAGCCACCAAACCTGCA	This study
	5 RACE	(Rev) AATCCAGTTCTCCAATGGC
	RT-PCR	(For) CTGCCTCCAAAAGGGGCAG	This study
		(Rev) TCAGGGATGCCAGGAACTGC
	qRT-PCR	(For) CCAGCCACCAAACCTGCAG	This study
		(Rev) GAGCCTGTTGTCAGTGTCC
DuIL-17A	qRT-PCR	(For) ATGTCTCCAACCCTTCGT	Kim et al. (2015)
		(Rev) CCGTATCACCTTCCCGTA
β-Actin	qRT-PCR	(For) GCTATGTCGCCCTGGATTTC	Liu et al. (2012)
		(Rev) CACAGGACTCCATACCCAAGAA

RT-PCR: reverse transcription-polymerase chain reaction; qRT-PCR: quantitative real-time polymerase chain reaction; RACE: rapid amplification of cDNA ends; DuIL-22: duck interleukin-22.