Research Article
Increased Circulating CD4+CXCR5+ Cells and IgG4 Levels in Patients with Myelodysplastic Syndrome with Autoimmune Diseases
Figure 1
Increased frequency of CD4+CXCR5+ lymphocytes in the peripheral blood of newly diagnosed MDS patients with autoimmune disease (MDS-AI). (a) The gate of lymphocyte population in the peripheral blood with forward scatter (FSC) and side scatter (SSC). (b) Representative dot plots from flow cytometric (FACS) analyses showing the CD4+CXCR5+ lymphocyte frequency among peripheral blood obtained from newly diagnosed MDS-AI (B1) and newly diagnosed MDS patients without autoimmune disease (MDS-NAI) (B2). (c) Representative dot plots from FACS analyses showing the PD1 expression of CD4+CXCR5+ lymphocyte frequency among peripheral blood obtained from MDS-AI (C1) and MDS-NAIs (C2). (d) Frequency of CD4+CXCR5+ lymphocytes from peripheral blood was compared between MDS-AI () and MDS-NAI (). (e) Frequency of CD4-CXCR5+ lymphocytes from peripheral blood were compared between MDS-AI () and MDS-NAI ().(f) Frequency of PD1 on CD4+CXCR5+ lymphocytes from peripheral blood was compared between MDS-AI () and MDS-NAI (). The bars represent the standard error of the mean.