Immunization with CENP-C Causes Aberrant Chromosome Segregation during Oocyte Meiosis in Mice

Figure 2

Cellular existence of the CENP-C antibody in mouse oocytes. Mouse oocytes from the control group and experimental group were immunolabeled with FITC-conjugated goat anti-mouse IgG for the CENP-C antibody (green) and counterstained with DAPI for chromosomes (blue). We detected whether the anti-CENP-C antibody was in oocytes at the GV stage and at the GVBD stage to ensure the antibody’s entrance into the cell before metaphase. The CENP-C antibody found in GV oocytes (b) and GVBD oocytes (c) from the experimental group. The CENP-C antibody was not detected in GV oocytes from the control group. Images were captured using a 20x objective lens and 10x eyepiece. Scale bar: 20 μm.