Immunization with CENP-C Causes Aberrant Chromosome Segregation during Oocyte Meiosis in Mice

Figure 6

Effects of the CENP-C antibody on spindle assembly and chromosome alignment in MII oocytes. Control and experimental oocytes were stained with the tubulin antibody to visualize spindles (green) and counterstained with DAPI to visualize chromosomes (blue). Control oocytes present a bipolar barrel-shaped spindle and well-aligned chromosomes on the metaphase equator (a), whereas spindle defects and chromosome misalignment were observed in experimental oocytes (b, c). Images were captured using a 40x objective lens and 10x eyepiece. Scale bar: 20 μm.