Propofol Suppresses Microglia Inflammation by Targeting TGM2/NF-κB Signaling

Figure 2

Knockdown or pharmacological inhibition of TGM2 suppresses activation of BV2 microglia. (a) Western blotting analysis of the knockdown efficiency of TGM2 in LPS-primed BV2 cells; β-actin was loaded as a control. (b) The effects of TGM2 knockdown on the mRNA expression of inflammatory genes (Il1b, IL6, Tnf, Ptgs2, and Nos2) in LPS-primed BV2 cells were analyzed by real-time qPCR analysis (). (c) The effects of TGM2 knockdown on the level of inflammatory cytokines (IL-1β, IL-6, and TNF-α) in conditioned medium from LPS-primed BV2 cells were analyzed by ELISA (). (d) The mRNA expression of inflammatory genes (Il1b, IL6, Tnf, Ptgs2, and Nos2) in LPS-primed BV2 cells with or without GK921 treatment was analyzed by real-time qPCR analysis (). (e) The level of inflammatory cytokines (IL-1β, IL-6, and TNF-α) in conditioned medium from LPS-primed BV2 cells with or without GK921 treatment was analyzed by ELISA (). , , and .

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