Propofol Suppresses Microglia Inflammation by Targeting TGM2/NF-κB Signaling

Figure 3

TGM2 promotes BV2 activation independent of its transamidase-activity. (a) Western blotting analysis of the overexpression efficiency of TGM2-WT, TGM2-C277S, and TGM2-R580K in BV2 cells. (b) The effects of TGM2-WT, TGM2-C277S, and TGM2-R580K on the mRNA expression of inflammatory genes (Il1b, IL6, Tnf, Ptgs2, and Nos2) in BV2 cells were analyzed by real-time qPCR analysis (). (c) The effects of TGM2-WT, TGM2-C277S, and TGM2-R580K on the level of inflammatory cytokines (IL-1β, IL-6, and TNF-α) in conditioned medium from BV2 cells were analyzed by ELISA (). , , and .

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