Propofol Suppresses Microglia Inflammation by Targeting TGM2/NF-κB Signaling

Figure 4

TGM2 activates NF-κB signaling to promote microglial activation. (a) The effects of TGM2 knockdown on the activation of NF-κB signaling in LPS-primed BV2 cells were analyzed by Western blotting. (b) The effects of TGM2 inhibition with GK921 on the activation of NF-κB signaling in LPS-primed BV2 cells were analyzed by Western blotting. (c) The effects of TGM2 knockdown on the NF-κB activity in LPS-primed BV2 cells were analyzed by luciferase reporter assay. (d) The effects of TGM2 inhibition with GK921 on the NF-κB activity in LPS-primed BV2 cells were analyzed by luciferase reporter assay. (e) The effects of Bay11-7082 (10 μM) on the mRNA expression of inflammatory genes (Il1b, IL6, Tnf, Ptgs2, and Nos2) in LPS-primed BV2 cells were analyzed by real-time qPCR analysis (). (f) The effects of Bay11-7082 (10 μM) on the level of inflammatory cytokines (IL-1β, IL-6, and TNF-α) in conditioned medium from LPS-primed BV2 cells were analyzed by ELISA (). and .

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