lncRNA OIP5-AS1 Suppresses Cell Proliferation and Invasion of Endometrial Cancer by Regulating PTEN/AKT via Sponging miR-200c-3p

Figure 2

Upregulation of lncRNA OIP5-AS1 inhibited the proliferation of EC cell in vitro. (a) qRT-PCR was conducted to evaluate the expression of OIP5-AS1 in EC cell lines. After being transfected, OIP5-AS1 was significantly upregulated in the HEC-1A cell line compared with the empty vector group. Meanwhile, OIP5-AS1 was reduced in the KLE cell line by transfecting with the anti-OIP5-AS1 sequence. . (b) CCK8 assay was used to measure cell vitality of HEC-1-A and KLE cells after transfection for 12, 24, 48, and 72 hours. (c) EDU staining was applied to label the DNA synthesis in HEC-1-A and KLE cells, which were transfected with OIP5-AS1 and its interfering RNA. Green represents EDU-positive cells; blue represents the cell nucleus. Scale bar 100 μM. (d) Statistical result of EDU-positive cells. (e, f) Cell cycle was determined by flow cytometry. (g) Western blot analysis of cell cycle-related protein Cyclin D and p27 protein in the presence and absence of OIP5-AS1. Error bars represent the of at least three independent experiments. , , and .

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