Research Article

lncRNA OIP5-AS1 Suppresses Cell Proliferation and Invasion of Endometrial Cancer by Regulating PTEN/AKT via Sponging miR-200c-3p

Figure 3

Overexpression of lncRNA OIP5-AS1 could promote the apoptosis and restrain EC cells’ tumorigenic potential. (a) Measurement of apoptotic cells with OIP5-AS1 overexpression by FACS assay and calculated percentage of apoptotic cells. and . (b) Western blot analysis of cell death-related protein Bcl-2 and cleaved-caspase3, and the internal control is GAPDH. (c) Colony formation assay detected cell proliferation in OIP5-AS1-upregulated HEC-1-A cells and OIP5-AS1-downregulated KLE cells. (d, e) Transwell assay to test the invasion ability of HEC-1-A and KLE by altering the level of OIP5-AS1. . (f) Wound healing assay to identify the migration capacity of two EC cells after transfecting the OIP5-AS1 vector and Si-OIP5-AS1 for 48 hours. .
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