lncRNA OIP5-AS1 Suppresses Cell Proliferation and Invasion of Endometrial Cancer by Regulating PTEN/AKT via Sponging miR-200c-3p

Figure 5

OIP5-AS1 could regulate PTEN’s expression via sponging miR-200c-3p. (a) Predictive binding sequence of miR-200c-3p and OIP5-AS1 by starBase 3.0. (b) Dual-luciferase reporter assay showed that miR-200c-3p mimics reduced fluorescence intensity of OIP5-AS1-WT, while having no influence in OIP5-AS1-Mut. (c) The level of miR-200c-3p was detected using qPCR assay when altering OIP5-AS1. (d) Predictive binding sequence of miR-200c-3p and PTEN by starBase 3.0. (e) Dual-luciferase reporter assay showed that miR-200c-3p could affect PTEN-3-UTR WT but not PTEN-3-UTR Mut. (f, g) The transcriptional level of PTEN was measured using a qPCR assay while affecting miR-200c-3p and OIP5-AS1.

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