lncRNA OIP5-AS1 Suppresses Cell Proliferation and Invasion of Endometrial Cancer by Regulating PTEN/AKT via Sponging miR-200c-3p

Figure 6

PTEN controls the progression of endometrial cancer by acting on a ceRNA of PTEN by sponging miR-200c-3p. (a) CCK8 assay was conducted to assess miR-200c-3p’s influence on the HEC-1-A cell. (b, c) EDU staining images revealed that miR-200c-3p could promote proliferation, and coexpressing OIP5-AS1 could alleviate the effect caused by miR-200c-3p. (d) Transwell assay indicated that miR-200c-3p could facilitate tumor invasion. (e) Western blot of PTEN and AKT when miR-200c-3p and OIP5-AS1 are altered. (f) Data mining using the TCGA database exhibited a positive correlation between PTEN and OIP5-AS1. (g) Western blot assay proved the OIP5-AS1 overexpression upregulation of the level of PTEN in the HEC1A cell line.

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