Deficiency of IKKα in Macrophages Mitigates Fibrosis Progression in the Kidney after Renal Ischemia-Reperfusion Injury

Figure 5

The difference between macrophages of WT mice (WT MΦs) and Mac IKKα−/− mice (KO MΦs). Macrophages were induced by injecting thioglycollate broth into the peritoneal cavity of mice for 3 days. (a) The percentage of CD14+ and F4/80+ KO MΦs was smaller than that of WT MΦs assessed by flow cytometry analysis. (b) Representative flow cytometry results showed that the proportion of CD206+ and F4/80+ KO MΦs was smaller than that of WT MΦs. (c) Representative images and quantification of Western blot analyses for expression of iNOS and TNF-α as M1 markers and Arg-1 and IL-10 as M2 markers. (d) There was no significant difference in the expression of iNOS and TNF-α, while the WT MΦs produced more Arg-1 and IL-10 compared with KO MΦs. Data are presented as the . versus Mac IKKα−/− mice; , independent -test. (e) The mRNA abundance for iNOS, TNF-α, Arg-1, and IL-10 in monocytes and macrophages isolated from WT mice or Mac IKKα−/− mice was assessed; data are presented as the . versus Mac IKKα−/− mice; , independent -test.

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