Deficiency of IKKα in Macrophages Mitigates Fibrosis Progression in the Kidney after Renal Ischemia-Reperfusion Injury

Figure 6

The amount and distribution of M2 macrophages in the kidney after IRI. Mice underwent unilateral renal pedicle clamping for 45 minutes followed by reperfusion. (a) Representative images of dual immunofluorescence staining for F4/80 (green) and CD206 (red) in kidney sections at day 3 after IRI. The proportion of F4/80+CD206+ macrophages in WT kidneys was larger than in Mac IKKα−/−kidneys. (b) Dual immunofluorescence staining for F4/80 (green) and CD206 (red) in kidney sections at day 7 after IRI. The cell density of F4/80+CD206+ macrophages in kidney sections was lower compared with the IRI kidney sections at day 3, while more F4/80+CD206+ macrophages can be seen in WT kidneys than Mac IKKα−/− kidneys. . Magnification, ×400. (c) Quantitative analysis for F4/80+CD206+ macrophages in kidney tissues. White arrows indicate macrophages positive for costaining. versus Mac IKKα−/− mice; , independent -test.

(a)

(b)

(c)