lncRNA DCST1-AS1 Facilitates Oral Squamous Cell Carcinoma by Promoting M2 Macrophage Polarization through Activating NF-κB Signaling

Figure 5

Blocking the NF-κB pathway inhibited the progression of OSCC cells and M2-like polarization of macrophages triggered by overexpression of DCST1-AS1. SCC-9 cells transfected with oe-DCST1-AS1 were treated with or without the NF-κB antagonist. (a) SCC-9 cell viability evaluated using the CCK-8 assay. (b) Transwell assay was used to assess SCC-9 cell migration and invasion capacity. THP-1-differentiated macrophages transfected with oe-DCST1-AS1 were incubated with or without the NF-κB antagonist. (c, d) Flow cytometry analysis of M1 markers (CD11c and CD80) and M2 markers (CD11b and CD206). .

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