SLAM/SAP Decreased Follicular Regulatory T Cells in Patients with Graves’ Disease

Figure 2

The role of SLAM on circulating CD4⁺CXCR5⁺ T cells. Addition of 0.5 μg/ml anti-CD3 mAb and 2 μg/ml anti-SLAM mAb to CD4⁺CXCR5⁺ T cells, followed by determination of the percentage of (a) Foxp3⁺ cells, (d) INF-γ⁺ cells, (e) IL-4⁺ cells, and (f) IL-17⁺ cells. (b) Addition of 0.5 μg/ml anti-CD3 mAb and 2 μg/ml anti-SLAM mAb to CD4⁺ T cells and detection of the percentage of Foxp3⁺ cells. (c) Addition of 0.5 μg/ml anti-CD3 mAb with 0.125 μg/ml, 0.5 μg/ml, and 2 μg/ml anti-SLAM mAb to CD4⁺CXCR5⁺ T cells and detection of the percentage of Foxp3⁺ cells. Each experiment was repeated three times; ; ; ns: no significant differences.

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