Research Article
Therapeutic Mechanism and Effect of Camptothecin on Dextran Sodium Sulfate-Induced Ulcerative Colitis in Mice
Table 2
Primer sequences used to target TNF-α, IL-1β, IL-6, and β-actin.
| | Item | Primer | Amplicon length (bp) |
| | TNF-α (sense) | 5-ACGGCATGGATCTCAAAGAC-3 | 116 | | TNF-α (antisense) | 5-GTGGGTGAGGAGCACGTAGT-3 | | IL-1β (sense) | 5-GCTGCTTCCAAACCTTTGAC-3 | 121 | | IL-1β (antisense) | 5-AGCTTCTCCACAGCCACAAT-3 | | IL-6 (sense) | 5-CCGGAGAGGAGACTTCACAG-3 | 134 | | IL-6 (antisense) | 5-CAGAATTGCCATTGCACAAC-3 | | iNOS (sense) | 5-GAACTGTAGCACAGCACAGGAAAT-3 | 158 | | iNOS (antisense) | 5-CGTACCGGATGAGCTGTGAAT-3 | | COX-2 (sense) | 5-CAGTTTATGTTGTCTGTCCAGAGTTTC-3 | 127 | | COX-2 (antisense) | 5-CCAGCACTTCACCCATCAGTT-3 | | β-Actin (sense) | 5-GTCAGGTCATCACTATCGGCAAT-3 | 147 | | β-Actin (antisense) | 5-AGAGGTCTTTACGGATGTCAACGT-3 |
|
|
