Selective Cytotoxicity of Single and Dual Anti-CD19 and Anti-CD138 Chimeric Antigen Receptor-Natural Killer Cells against Hematologic Malignancies

Figure 4

Selective cytotoxicity of CD19-NK-92 and CD138-NK-92 cells toward various hematologic cancer cells. (a) Human CML-derived K562, BL-derived Raji, MM-derived H929 and RPMI-8226 cells, and ALL-derived REH were labeled with PKH67 dye; incubated with WT-NK-92, CD19-NK-92, or CD138-NK-92 cells at the  :  ratio of 1 : 5 for 4 h; and stained with Annexin V-PE and 7-AAD. Representative dot plots of Annexin V-PE versus 7-AAD in PKH67-labeled cells were shown. (b) Percentage of total cancer cell death was plotted. Data are (). and versus WT-NK-92 cells; two-sided Student’s -test.

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