Selective Cytotoxicity of Single and Dual Anti-CD19 and Anti-CD138 Chimeric Antigen Receptor-Natural Killer Cells against Hematologic Malignancies

Figure 7

Selective cytotoxicity of dual CD19/CD138-NK-92 cells toward various hematologic cancer cells. (a) Human CML-derived K562, ALL-derived REH, BL-derived Raji, and MM-derived H929 and RPMI-8226 cells were labeled with PKH67 dye, incubated with WT-NK-92 or dual-NK-92 cells at the  :  ratio of 1 : 5 for 4 h, and stained with Annexin V-PE. Representative flow cytometric dot plots of Annexin V-positive cells (red box) in PKH67-labeled cells were shown. (b) Percentage of cancer cell death was plotted. Data are (). and versus WT-NK-92 cells; two-sided Student’s -test.

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