Selective Cytotoxicity of Single and Dual Anti-CD19 and Anti-CD138 Chimeric Antigen Receptor-Natural Killer Cells against Hematologic Malignancies

Figure 8

Cytotoxicity of the mixture of CD19-NK-92 and CD138-NK-92 cells toward ALL cells expressing CD19 and, to a lesser extent, CD138 antigens. (a) Human ALL-derived REH cells were labeled with PKH67 dye, incubated with WT-NK-92 cells or the mixture CD19-NK-92 and CD138-NK-92 cells at various ratios (0 : 5˗5 : 1) at the final  :  ratio of 1 : 1 for 4 h, and stained with Annexin V-PE. Representative flow cytometric dot plots of Annexin V-positive cells (red box) in PKH67-labeled cells were shown. (b) Percentage of REH cell death was plotted. Data are (). versus WT-NK-92 cells; two-sided Student’s -test.

(a)

(b)