Neuromedin U Suppresses Collagen-Induced Arthritis through ILC2-Th2 Activation

Figure 2

NMU-23 induced ILC2 expansion and Th2 responses in the joint of arthritic mice. (a) Gating strategy for the identification of total ILCs (CD45⁺Lineage^-CD127⁺), ILC1 (NK1.1⁺T-bet⁺), ILC2 (KLRG1⁺ICOS⁺), and ILC3 (NK1.1^-RORγt⁺) in mesenteric lymph node (mLN) of arthritic mice. (b–d) The frequency of ILC2 (b), ILC1 (c), and ILC3 (d) in the joint, mLN, and spleen of arthritic mice. (e) Representative flow cytometry plot and quantification of eosinophils (CD45⁺SiglecF⁺) in the joint of arthritic mice. (f) The percentage of Th2 cells in mLN and spleen of arthritic mice. (g) Sera TNF-α, IL-4, IL-5, and IL-6 levels were determined by Luminex. Data are shown as (, , and determined by Student’s -test).

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