Granulocyte-Macrophage Colony-Stimulating Factor-Activated Neutrophils Express B7-H4 That Correlates with Gastric Cancer Progression and Poor Patient Survival

Figure 2

B7-H4 expression and activation of neutrophils are correlated in human GC environment. (a) Statistics analysis of the percentage of CD54⁺ neutrophils and B7-H4⁺ neutrophils in total neutrophils in each sample of patients with GC (). (b) Expression of molecules CD54 and B7-H4 on neutrophils. Color histograms represent staining of neutrophil activation marker CD54 and immunosuppressive molecule B7-H4; black: isotype control. (c) Statistics analysis of the number of CD54⁺ neutrophils and B7-H4⁺ neutrophils per million total cells in each sample of patients with GC (). (d) The correlations between CD54⁺ neutrophils and B7-H4⁺ neutrophils in GC tumors were analyzed. Results are expressed as the percentage of CD54⁺ neutrophils and B7-H4⁺ neutrophils in neutrophils or the number of CD54⁺ neutrophils and B7-H4⁺ neutrophils per million total cells in tumor tissues of patients with GC. The horizontal bars in (a) and (c) represent mean values. Each ring or dot in (a), (c), and (d) represents 1 patient. , , and ^n.s. for groups connected by horizontal lines.

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