Decreased Expression of MPC2 Contributes to Aerobic Glycolysis and Colorectal Cancer Proliferation by Activating mTOR Pathway

Figure 2

MPC2 suppressed CRC growth in vitro and in vivo. (a) The protein expression of MPC2 in seven CRC cell lines was detected using western blotting. (b) The inhibition efficiency in RKO cells (right) and HCT116 cells (left) using western blotting. (c) The inhibition efficiency in HCT116 (left) and RKO (right) cells showed by qRT-PCR (relative to shNC). (d) The viability of HCT116 (left) as well as RKO cell (right) transfected with shMPC2 or shNC detected with CCK-8 assay. (e) Colony formation ability of HCT116 and RKO cells through transfection by shNC or sh-MPC2. (f) Representative bioluminescence images of mice orthotopically injected with mcherry and luciferase-expressing HCT116 cells transfected with shNC and shMPC2 as well as vector and OE-MPC2. (g) The representative images in gross and weight of tumor mass from orthotopic mouse model among shNC, sh-MPC2, vector, and OE-MPC2 groups (Student’s -test). (h) Ki67’s expression in the orthotopic tumor tissues (shNC, sh-MPC2, vector, and OE-MPC2, ). , , , and .

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