Decreased Expression of MPC2 Contributes to Aerobic Glycolysis and Colorectal Cancer Proliferation by Activating mTOR Pathway

Figure 3

MPC2 expression suppressed glycolysis in CRC cells. (a) GSEA of MPC2 expression in CRC samples from the TCGA database. (b) Lactate production and glucose intake of CRC cells transfected with shNC or shMPC2 (relative to shNC). (c) Relative mRNA levels of glycolysis-related genes of HCT116 (left) as well as RKO cells (right) transfected with shNC or shMPC2 (relative to shNC). (d) Glycolytic function of HCT116 as well as RKO cells under the indicated treatments as measured by extracellular acidification rate (ECAR). 2-DG: 2-deoxyglucose (oxidative phosphorylation inhibitor). (e) Mitochondrial stress test of RKO cells (right) and HCT116 cells (left) and in MPC2 knockdown and/or overexpression under the indicated treatments as measured by oxygen consumption rate (OCR). A&R: actinomycin A (mitochondrial complex III inhibitor) and rotenone (the mitochondrial complex I inhibitor). , , , and .

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