Decreased Expression of MPC2 Contributes to Aerobic Glycolysis and Colorectal Cancer Proliferation by Activating mTOR Pathway

Figure 4

MPC2 reduced glycolysis level via mTOR pathway in CRC cells. (a) GSEA from CRC tissues from the TCGA database. (b) Western blotting images of phospho-mTOR (p-mTOR) and mTOR in HCT116 and RKO cells with shNC or shMPC2 transfection and/or rapamycin treatment (50 nmol/L). (c) Lactate production and glucose consumption of CRC cells with shNC or shMPC2 transfection and/or rapamycin. (d) Relative mRNA expression of glycolysis-related genes of HCT116 and RKO cells with shNC or shMPC2 transfection and/or rapamycin treatment. (e) Glycolytic function of RKO and HCT116 cells under the indicated treatments as measured by ECAR. (f) Mitochondrial stress test RKO cells (right) and HCT116 cells (left) under the indicated treatments as measured by oxygen consumption rate (OCR). , .

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