The Role of β-Catenin in Th1 Immune Response against Tuberculosis and Profiles of Expression in Patients with Pulmonary Tuberculosis

Figure 1

The genotypes of all mice identified by PCR and T cells frequency in both groups of mice by flow cytometry. (a) Mice’s genotypes were identified by PCR, and results were showed by 2.5% agarose gel electrophoresis. (b) The frequency of β-catenin⁺ cells of CD4⁺ T cells in lungs and spleens of Ctnnb1^-/- mice () and wild-type mice (); CD3⁺CD4⁺ T cells were shown in the lungs (c) and spleens (d) of Ctnnb1^-/- () and wild-type mice () infected with or without M. tuberculosis; CD3⁺CD8⁺ T cells were shown in the lungs (e) and spleen (f) of Ctnnb1^-/- () and wild type mice () infected with or without M. tuberculosis. The experiment had three independent rounds, and results were analyzed by the Student test. NTC, no template control. PBS, phosphate-buffered saline.

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