Cloning and Characterization of Immunological Properties of Haemophilus influenzae Enolase

Figure 5

Purification and identification of rNTHiENO. Protein was resolved by SDS-PAGE on 12% polyacrylamide gel and stained with Coomassie blue. (a) Lane M: molecular weight marker. Lane 1: E. coli Bl21 cell containing the pRSET-A::NTHiENO without IPTG induction. Lane 2: E. coli Bl21 cell containing the pRSET-A::NTHiENO with IPTG induction. Lane 3: insoluble pellet. Lane 4: washing material. Lane 5: the purified rNTHiENO. Identification of rNTHiENO by western blot using a monoclonal antibody anti-His Tag. (b) Lane M: molecular weight marker. Lane 1: pRSET-A::NTHiENO in E. coli BL21, noninduced. Lane 2: purified rNTHiENO. The band corresponding to rNTHiENO (52.0 kDa) is indicated with an arrow.

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