IL-13 Augments Histone Demethylase JMJD2B/KDM4B Expression Levels, Activity, and Nuclear Translocation in Airway Fibroblasts in Asthma

Figure 3

JMJD2B and its downstream target levels after IL-13 stimulation in normal and asthmatic fibroblasts. (a) qRT-PCR analysis of JMJD2B expression levels upon IL-13 stimulation in normal and asthma fibroblasts. (b) qRT-PCR analysis of JMJD2B expression levels comparing fibroblasts with or without IL-13 stimulation in normal and asthma fibroblasts. (c) Western bolt analysis showing JMJD2B and trimethylated histones H3 lysine residue at K36 protein levels in the fibroblasts upon IL-13 stimulation. (d) Calculated fold change in protein-expression levels comparing fibroblasts with or without IL-13 stimulation in normal and asthma fibroblasts based on two separate experiments. Graphical data are represented as . , determined using unpaired two-tailed Student -test. Representative immunoblots depicting protein levels normal and asthma fibroblasts where β-actin was used as loading control.

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