Tissue-Resident Memory-Like CD8⁺ T Cells Exhibit Heterogeneous Characteristics in Tuberculous Pleural Effusion

Figure 4

Heterogeneous Granzyme B expression by distinct CD8⁺ T_RM-like cells from resting PFMCs. PFMCs were stimulated with BCG plus anti-CD28 for 12 hours in the presence of BFA, the coexpression of IFN-γ, CD69, and CD103 was detected, and the representative dot plots were shown (a) (). After stimulation with BCG plus anti-CD28, the frequencies of CD69^-CD103^-, CD69⁺CD103^-, CD69⁺CD103⁺, and CD69^-CD103⁺ cells in IFN-γ⁺CD8⁺ T cells were detected; the (b) representative dot plots and (c) statistical data were shown (). The coexpression of Granzyme B and CD45RO, CD103, or CD69 was observed; the (d) representative dot plots were shown, and the correlations between Granzyme B and CD45RO, CD103, or CD69 in resting PFMCs were analyzed () (e). The expression of Granzyme B by different CD45RO⁺CD8⁺ T subsets distinguished based on CD103 and CD69 expression was analyzed; the (f) representative dot plots and (f) histogram graphs and (g) statistical data were shown (). , , and .

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