High Expression of VSTM2L Induced Resistance to Chemoradiotherapy in Rectal Cancer through Downstream IL-4 Signaling

Figure 3

Overexpression of VSTM2L induced resistance to CRT in colorectal cancer cells in vitro. (a, b) The endogenous mRNA and protein levels of VSTM2L expression in 5 colorectal cancer cell lines. (c, d) The transfected effects of VSTM2L overexpression in LS.174T and SW837 cells were analyzed by qRT-PCR and western blot analysis. GAPDH was used as the loading control. and . (e) The effect of overexpression of VSTM2L in LS.174T and SW837 cells treated with CRT, as determined by the CCK-8 assay. The results are shown as the (), , ^ns. (f, g) The effect of overexpression of VSTM2L in CRC cells treated with CRT on colony formation assays. The results are shown as (), , and ^ns. (f, g) The effect of overexpression of VSTM2L in CRC cells treated with CRT on γ-H₂AX expression to evaluate the ability of DNA damage. The results are shown as (), , , and ^ns. (j, k) VSTM2L overexpression in CRC cells treated with CRT significantly inhibited cell apoptosis, as determined by Annexin V-APC/PI staining and flow cytometry. and ^ns.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

(k)