Antigen-Specific Tissue-Resident Memory T Cells in the Respiratory System Were Generated following Intranasal Vaccination of Mice with BCG

Figure 2

The proportions of CD3⁺, CD4⁺, and CD8⁺ T cells in the lavage fluids, nasal mucosa, trachea, lungs, and blood. CD45⁻CD3⁺ T cells (noncirculating T cells) and CD45⁺CD3⁺ T cells (circulating T cells) from lavage fluid, nasal mucosa, trachea, and lung tissues were distinguished by tail vein injection with fluorochrome-conjugated CD45 antibody. (a) Live and singlet CD3 lymphocytes from nasal mucosa, trachea, lung tissues and blood were gated and subsequently analyzed on the percentage of labeled CD45⁺ T cells by flow cytometry. (b) Live and singlet CD45⁻ lymphocytes from lavage fluid, nasal mucosa, trachea, and lung tissues and CD45⁺ lymphocytes from blood were gated and subsequently analyzed on CD3⁺, CD4⁺, and CD8⁺ T cells by flow cytometry. The tissue cells of the respiratory system were shown in the representative pseudocolor graphs. (c) The statistical results of noncirculating CD3⁺, CD4⁺, and CD8⁺ T cells in lavage fluids, nasal mucosa, trachea, and lungs and circulating CD3⁺, CD4⁺, and CD8⁺ T cells in blood were shown as of eight independent experiments. Statistical significance was determined with one-way ANOVA for multiple comparisons. and ; ns: no significance.

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