Antigen-Specific Tissue-Resident Memory T Cells in the Respiratory System Were Generated following Intranasal Vaccination of Mice with BCG

Figure 3

The tissue-resident memory T cells including CD4⁺ and CD8⁺ T cells in the lavage fluids, nasal mucosa, trachea, and lungs. (a) Live and singlet T_RM cells from lavage fluid, nasal, trachea, and lung tissues were gated on CD3⁺CD44^high, CD4⁺CD44^high, and CD8⁺CD44^high T cells and subsequently analyzed by flow cytometry. (b) The statistical results of CD69⁺ T, CD103⁺ T, and CD69⁺CD103⁺ T cells on CD3⁺CD44^high, CD4⁺CD44^high, and CD8⁺CD44^high T cells in the lavage fluids, nasal mucosa, trachea, and lungs were shown as of six independent experiments. Statistical significance was determined with Tukey’s multiple comparisons test. , , and ; ns: no significance.

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