Antigen-Specific Tissue-Resident Memory T Cells in the Respiratory System Were Generated following Intranasal Vaccination of Mice with BCG

<div>The expression of IFN-<i>γ</i> was higher in CD69<sup>+</sup> and CD103<sup>−</sup> T cells than CD69<sup>−</sup> and CD103<sup>+</sup> T cells when stimulated by PMA and ionomycin. (a) Live and singlet T<sub>RM</sub> cells from lavage fluid, nasal, trachea, and lung tissues were gated on CD3<sup>+</sup>CD44<sup>high</sup>, CD4<sup>+</sup>CD44<sup>high</sup>, and CD8<sup>+</sup>CD44<sup>high</sup> T cells and subsequently analyzed on the expression of IFN-<i>γ</i> by flow cytometry. All cells were stimulated for 6 hrs with PMA and ionomycin in the presence of BFA in flow tubes. (b) The cells were detected for the frequency of INF-<i>γ</i> in CD69<sup>−/+</sup> or CD103<sup>−/+</sup> cells by FACS. 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Journal of Immunology Research

fig5

Figure 5

Figure 5: Antigen-Specific Tissue-Resident Memory T Cells in the Respiratory System Were Generated following Intranasal Vaccination of Mice with BCG 