Antigen-Specific Tissue-Resident Memory T Cells in the Respiratory System Were Generated following Intranasal Vaccination of Mice with BCG

Table 4

The expression of IFN-γ in T_RM subsets of respiratory tissues before and after immunization.

Subset of TRM cells	Sample	Medium group (mean %)	BCG group (mean %)	value
IFN-γ in CD69^+CD103^+ T cells	Lavage fluid	0.1825	5.055	0.0003
	Nasal mucosa	0.6025	2.71	0.0033
	Trachea	0.6	5.23	0.00008
	Lung	0.4325	2.705	0.0001
IFN-γ in CD69^+CD103^-T cells	Lavage fluid	0.2925	9.5875	0.0002
	Nasal mucosa	0.3365	4.405	0.00009
	Trachea	0.695	8.55	0.00007
	Lung	0.6775	4.27	0.00008
IFN-γ in CD69^-CD103^-T cells	Lavage fluid	0.415	3.625	0.0005
	Nasal mucosa	0.3025	2.125	0.0021
	Trachea	0.545	2.1725	0.001
	Lung	0.595	1.79	0.0017
IFN-γ in CD69^-CD103^+T cells	Lavage fluid	0.21	0.4875	0.104
	Nasal mucosa	0.545	0.4425	0.526
	Trachea	0.6625	0.7725	0.6259
	Lung	0.515	0.8275	0.2016

BCG induced the expression of IFN-γ by tissue-resident memory T cells in the lavage fluids, nasal mucosa, trachea, and lungs. Respiratory system tissues of lavage fluid, nasal mucosa, trachea, lung, and blood cells were stimulated for 12 hrs with or without BCG plus anti-CD28 in the presence of BFA in flow tubes. The expression of antigen specific IFN-γ in the four groups of CD69^-/+CD103^-/+ T cells on CD44^highCD3⁺ T cells were analyzed. The statistical results of the expression of antigen-specific IFN-γ in the four groups of CD69^−/+CD103^−/+ T cells as mean. The significance was compared with two-way ANOVA. and ; : no significance.