Identification of Immune-Related Prognostic Biomarkers Associated with HPV-Positive Head and Neck Squamous Cell Carcinoma

Figure 10

Overexpression of IGSF5 inhibits the proliferation of HNSCC cells. (a) IGSF5 had lower expression in HNSCC samples than in matched normal tissues from the TCGA database (, ). (b) IGSF5 in HPV-positive HNSCC tissues from the GSE65858 cohort was upregulated compared with that in HPV-negative tissues. (c) The expression of IGSF5 was detected by Western blot, and cells transfected with the IGSF5 overexpression plasmid differed significantly between the IGSF5 overexpression (pCDNA-IGSF5) and control (vector) groups in Hep-2 and TU212 cells. GAPDH was used as an internal control. (d) Reduction in the proliferation ability of pCDNA-IGSF5 Hep-2 and TU212 cells compared with the control (vector) cells by the CCK8 assay. (e, f) Reduction in colony formation ability of pCDNA-IGSF5 Hep-2 and TU212 cells compared with the control (vector) cells by a colony formation assay. The bar graph indicates the number of colonies. (g, h) Cell apoptosis of pCDNA-IGSF5 Hep-2 and TU212 cells compared with the control (vector) was analyzed by flow cytometry. The rate of cell apoptosis is shown in the graphs. The results are presented as the and are representative of at least three independent experiments. , , , and . ns: .

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