Involvement of CD26 in Differentiation and Functions of Th1 and Th17 Subpopulations of T Lymphocytes

Figure 1

The activation and proliferation of T lymphocyte after stimulation. After 72 h of the stimulation with immobilized anti-CD3 mAb, the activation of lymphocytes was determined by the measurement of the expression of T lymphocyte activation markers (CD69, CD71, CD25, and CD26). The proliferation of lymphocytes was analyzed by CFSE assay. PBS-treated cells were used as controls. (a) Percentages of CD26⁺-HPBLs in the control group and the stimulated group. (b) Percentages of CD69⁺-, CD71⁺-, and CD25⁺-HPBLs in the control group and the stimulated group. Data represented from a minimum of 5 independent experiments with at least 5 healthy donor HPBL samples, and each experiment was repeated more than 3 times. values were calculated with a chi-square test. (c) Histogram of the proliferated generations of lymphocytes after stimulation by immobilized anti-CD3 mAb (anti-CD3) or only PBS as control (PBS) for three days. The shaded histogram represents the original generation (0g) of the PBS control group at day 3. The hollow histogram indicates the increased 5 generations (1g, 2g, 3g, 4g, and 5g) of the stimulated group three days after stimulation. (d) The dot plots show the proliferated generations of lymphocytes analyzed by flow cytometry.

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