Involvement of CD26 in Differentiation and Functions of Th1 and Th17 Subpopulations of T Lymphocytes

Figure 6

Percentage of cells secreting different cytokines in the CD26^low and CD26^high groups. After separation, the cells in the CD26^low group and the CD26^high group were labeled with different monoclonal antibodies against cytokines or surface markers at 4°C for 30 min and then measured by flow cytometry. (a) The percentages of cells secreting Th1 and Th2 typical cytokines in the CD26^low and CD26^high groups. (b) The percentages of cells secreting Th17 typical cytokines or expressing Th17 typical biomarkers in the CD26^low and CD26^high groups. (c) Overlay histograms demonstrate the relative expression of cells secreting different cytokines in the CD26^low and CD26^high groups. The black line indicates the values of CD26^low group cells while the color lines indicated the values of CD26^high group cells. (d) The percentages of cells expressing Tregs typical biomarkers in the CD26^low and CD26^high groups. Data represented from a minimum of five independent experiments with at least five healthy donor HPBL samples, and each experiment was repeated more than three times.

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