Involvement of CD26 in Differentiation and Functions of Th1 and Th17 Subpopulations of T Lymphocytes

Figure 7

Coexpression of CD26 with each of the Th1 or Th17 typical cytokines or surface markers in the cells of the CD26^low and CD26^high groups. Lymphocytes were harvested at 72 h after stimulation and were double-stained with the FITC-conjugated anti-CD26 mAb and PE-conjugated anti-IL-2, anti-IFN-γ, anti-IL-17, anti-IL-6, anti-IL-22, or anti-IL-23R mAb. (a) Percentages of cells coexpressing CD26 with each of the Th1 typical cytokines (IL-2 or IFN-γ), Th17 typical cytokines (IL-6, IL-17, and IL-22), or Th17 typical surface marker (IL-23R) in the CD26^low and CD26^high groups. Data represented from a minimum of five independent experiments with at least five healthy donor HPBL samples, and each experiment was repeated more than three times. (b) Coexpression of CD26 with Th1 or Th17 typical biomarkers was observed by fluorescence microscopy. Images were made at ×600 magnifications. Coexpression of CD26 with IL-2, IFN-γ, IL-17, IL-22, or IL-23R in some lymphocytes indicated by the merged images.

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