Invariant Natural Killer T Cells as Key Players in Host Resistance against Paracoccidioides brasiliensis

Figure 1

The invariant Natural Killer T cells drive P. brasiliensis killing by macrophages and respond for the innate source of IFN-γ upon fungal infection. Thioglycolate-elicited peritoneal MΦ from C57Bl/6 mice were infected with Pb18 yeast (1 : 10) for 2 h. After washing, splenocytes (Spln) from WT, CD1d^-/-, or Jα18^-/- C57BL/6 mouse strains were added to the MΦ culture (10 : 1) for 48 h. After this period, supernatant was collected, and adherent cells were lysed with distilled water for fungal recovery. (a) Number of viable cell yeast obtained by colony-forming units assay (CFU). (b) NO levels in culture supernatants. (c) IFN-γ levels in the culture supernatants (ND = not detected). Data represent the of quintuplicate samples from 1 of 2 independent experiments (a, b) and of 1 independent experiment (c). ; ; .

(a)

(b)

(c)