Evaluation of the PEΔIII-LC3-KDEL3 Chimeric Protein of Entamoeba histolytica-Lectin as a Vaccine Candidate against Amebic Liver Abscess

Figure 8

(a) Macroscopic analysis of amebic liver abscess formation. (A, B) No abscesses were detected in the liver of uninfected hamsters. (C, D) The liver of the sham group showed the characteristic ALA lesions. (E, F) The 50 μg group exhibited smaller lesions on day 7 than day 4 than sham. (G, H) A single small lesion is seen in the 75 μg group (arrows). (I, J) No lesion was detected in the liver of the 100 μg group. (b) Light microscopy of paraffin histological technique of amoebic liver abscess. Original magnification 400x. (A) In the intact hamsters, normal architecture was found in the liver tissue. (B, C) In two of the infected groups, sham and vaccinated with 50 μg, a necrotic area was observed in the liver parenchyma, accompanied by inflammatory infiltrate (arrows, asterisk). (D) In the 75 μg group, the parenchyma displayed no tissue necrosis (arrowhead). (E) In the 100 μg group, the parenchyma was similar to the intact animals, and there was no tissue necrosis or inflammation (arrows, cross).

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