MicroRNA-34a Promotes EMT and Liver Fibrosis in Primary Biliary Cholangitis by Regulating TGF-β1/smad Pathway

Figure 1

TGIF2 was the target gene of miR-34a. (a) The predicted two binding sequence of miR-34a on 3-UTR of TGIF2 and their mutations. (b) TGIF2 mRNA level (miR-34a mimics vs. NC, ; miR-34a mimics vs. inhibitor, ; and miR-34a inhibitor vs. NC, ). (c) Relative TGIF2 protein expression (miR-34a mimics vs. NC, ; miR-34a mimics vs. inhibitor, ; and miR-34a inhibitor vs. NC, ). (d) Relative luciferase activity of wild-type TGIF2 (L/TGIF2 WT+mimics vs. L/TGIF2 WT+NC, and L/TGIF2 WT+mimics vs. L/TGIF2 WT, ). (e) Relative luciferase activity of three types mutations of TGIF2 (L/TGIF2 mut1+mimics vs. L/TGIF2 mut1+NC, ; L/TGIF2 mut2+mimics vs. L/TGIF2 mut2+NC, ; and L/TGIF2 mut3+mimics vs. L/TGIF2 mut3+NC, ns).

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