Circular RNA FOXP1 Induced by ZNF263 Upregulates U2AF2 Expression to Accelerate Renal Cell Carcinoma Tumorigenesis and Warburg Effect through Sponging miR-423-5p

Figure 4

U2AF2 is a downstream target of miR-423-5p. (a) Downstream targets for miR-423-5p were predicted using the DIANA tool microT (http://diana.imis.athena-innovation.gr/DianaTools/index.php?r=microT_CDS/index), PITA (https://genie.weizmann.ac.il/pubs/mir07/mir07_data.html), and TargetScan (http://www.targetscan.org/mamm_31/) datasets with CLIP Data: strict stringency (≥5), and Degradome Data: medium stringency (≥2); results were presented by Venn diagram. (b, c) Putative targets of miR-423-5p were examined by biotinylated RNA pull-down in ACHN (b) and 786-O (c) cells; results were measured using the qRT-PCR assay. (d) Predicted U2AF2 wild type (wt) or mutant type (Mut) bind sequences with miR-423-5p. (e–g) Relative luciferase activities in indicated reporter vector-treated 293T (e), ACHN (f), and 786-O (g) cells were measured. (h) Expression levels of U2AF2 in thirty pairs of RCC tissues were measured by qRT-PCR assay. (i, j) Expression levels of U2AF2 mRNA (h) or protein (i) in miR-423-5p overexpression ACHN and 786-O cells were measured by qRT-PCR and western blot. All assays were conducted in triplicate, and .

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