Circular RNA FOXP1 Induced by ZNF263 Upregulates U2AF2 Expression to Accelerate Renal Cell Carcinoma Tumorigenesis and Warburg Effect through Sponging miR-423-5p

Figure 6

Expression of circFOXP1 is induced by ZNF263. ACHN and 786-O cells were infected with Si-NC, Si-ZNF263#1, and Si-ZNF263#2. (a) Expression of ZNF263 in treated as indicated cells was measured using qRT-PCR. (b) Expression of circFOXP1 in ZNF263 knockdown cells was measured by qRT-PCR. (c) Bioinformatically predicted binding sequence of ZNF263. (d) Bioinformatically predicted binding site of FOXP1 promoter splices. (e, f) AGO2-RIP was conducted to verify the binding ability between ZNF263 and FOXP1 promoter splices in ACHN (e) and 786-O (f). (g–j) Luciferase reporter assay was conducted to confirm the interaction between FOXP1 promoter splices (P3 and P4) and ZNF263 in ACHN (g and i) and 786-O cells (h and j). All experiments were performed in triplicate, , , and .

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