Neutrophils Promote Larynx Squamous Cell Carcinoma Progression via Activating the IL-17/JAK/STAT3 Pathway

Figure 1

LSCC cells enhanced the activation and mobility of neutrophils. (a) Neutrophils were cultured with four different mediums: RPMI-1640 with 10% FBS (CTL), RPMI-1640 with 10% FBS and 1.25% DMSO (DMSO), conditioned medium from LSCC cells, or conditioned medium from LSCC cells with 1.25% DMSO. Six hours later, the neutrophils were collected. (b, c) qRT-PCR was used for CD11b level in neutrophils after treatment with Tu177- or Tu686-conditioned medium. (d, e) qRT-PCR was used for CD18 level in neutrophils after treatment with Tu177- or Tu686-conditioned medium. (f, g) qRT-PCR was used for MPO level in neutrophils after treatment with Tu177- or Tu686-conditioned medium. (h–j) Migration assays were carried out in neutrophils which were cocultured with Tu177- (i) or Tu686- (j) conditioned medium. The control medium was RPMI-1640 with 10% FBS. Data are shown of three independent experiments in triplicate () ().

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