Neutrophils Promote Larynx Squamous Cell Carcinoma Progression via Activating the IL-17/JAK/STAT3 Pathway

Figure 3

Neutrophils promoted LSCC progression through IL-17. (a) Neutrophil-associated inflammatory factor levels were detected in control medium and neutrophil conditioned medium by qRT-PCR. (b) ELISA was used for levels of IL-8, IL-10, IL-12, and IL-17 in control medium and neutrophil conditioned medium. (c, d) LSCC cells were cultured with control medium and neutrophil conditioned medium. The conditioned medium from neutrophils was added with or without 1 μM IL-17 antibody (Cosentyx). Growth rates were detected by CCK8 in Tu177 and Tu686 cells. (e, f) The upper chambers were added with LSCC cells, and the lower chambers were added with conditioned medium with or without 1 μM IL-17 antibody. Migration results of Tu177 and Tu686 were shown. (g, h) The upper chambers coated with Matrigel were added with LSCC cells, and the lower chambers were added with conditioned medium with or without 1 μM IL-17 antibody. Invasion results of Tu177 and Tu686 were shown. (i, j) EMT-related markers in Tu177 and Tu686 cells were detected.

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