Neutrophils Promote Larynx Squamous Cell Carcinoma Progression via Activating the IL-17/JAK/STAT3 Pathway

Figure 4

Neutrophils promoted LSCC progression through JAK/STAT3 activation. (a, b) Tu177 and Tu686 cells were cultured with the indicated medium. JAK and STAT3 activations were detected by western blot in Tu177 and Tu686 cells. (c, d) LSCC cells were treated with conditioned medium with or without the STAT3 inhibitor SH-4-54 (200 nM). We added DMSO equal to the volume of the SH-4-54 (dissolved in DMSO) in CM which we labeled as neutrophil CM+DMSO. Growth rates were detected by CCK8 in Tu177 or Tu686 cells. (e, f) LSCC cells were treated with conditioned medium with or without the STAT3 inhibitor SH-4-54 (200 nM). Migration results of Tu177 and Tu686 at 24 hours were shown. (g, h) LSCC cells were treated with conditioned medium with or without the STAT3 inhibitor SH-4-54 (200 nM). Invasion results of Tu177 and Tu686 at 24 hours were shown. Data are shown of three independent experiments in triplicate () ().

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