Neutrophils Promote Larynx Squamous Cell Carcinoma Progression via Activating the IL-17/JAK/STAT3 Pathway

Figure 5

Inhibition of the IL-17/JAK/STAT3 pathway effectively blocked LSCC progression. (a, b) 2 ng/mL of IL-17 was added into the medium, and then, the cells were treated with SH-4-54 (200 nM). CCK8 assays were performed in Tu177 and Tu686 cells at day 0, day 2, day 4, and day 6. (c, d) Migration results of Tu177 and Tu686 at 24 hours were shown. (e, f) Invasion results of Tu177 and Tu686 at 24 hours were shown. (g, h) Tu177 and Tu686 cells were treated with 2 ng/mL of IL-17 or 200 nM STAT3 inhibitor SH-4-54 for 24 hours. EMT-related markers in Tu177 and Tu686 cells were detected by western blot.

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